Nitrogen Isotope Separation with Displacement Chromatography Using Cryptand Polymer

Nitrogen Isotope Separation by Ion Exchange Chromatography

Enriched stable isotopes of many elements have been widely used in many aspects and such isotopes have been primarily used as the tracer for agricultural and biochemical studies and the availability of the isotopically labeled compounds have been now extensively increasing. Chemical exchange separation of isotopes is based on the equilibrium fractionation of isotopes between two phases, ion exc...

Polymer Separation by Size Exclusion Chromatography

Proteins are the essence of life processes. DNA contains the coded information for life, and is analogous to computer software, but it is the proteins that are analogous to the hardware, that actually carry out the job. Proteins have many roles, from catalysts (enzymes), through proteins that bind and interact with other molecules to control their behaviour, to structural and storage proteins w...

Ampholyte-displacement chromatography for the separation of serum proteins.

The antibody fraction (less than 6ml) was added to immobilized enzyme (2ml of packed gel). The antibodies were recycled for 2h and the unattached antibodies washed from the gel for 1 h. Specific antibodies were eluted by washing with citric acid (0.1 M, pH2.2). Experiments marked with an asterisk were performed simultaneously. The molecular weights of immunoglobulin G and creatine kinase were t...

Isotope Effects, Isotope Separation and Isotope Fractionation

Separation of Sedative – Hypnotic Drugs with Mixed Micellar Liquid Chromatography

Separation of ten sedative- hypnotic drugs was performed by RP-HPLC using mixed micellar mobile phase. Effect of temperature, type and amount of organic modifier in mobile phase on efficiency (N) and asymmetry factor (B/A) showed that, the appropriate conditions for a good separation were 35°C and 7% (V/V) butanol in mobile phase.  Variations of selectivity factor versus butanol concent...